ISO 16649-1:2018 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase^{[9][10][13][14][17][18][19][20]}. It is applicable to

- products intended for human consumption,

- products intended for feeding animals,

- environmental samples in the area of food production and food handling, and

- samples from the primary production stage such as animal faeces, dust, and swabs.