GSO ISO 12869:2022

Gulf Standard   Current Edition
Approved on  12 May 2022

Water quality — Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)

GSO ISO 12869:2022 Files

GSO ISO 12869:2022 Scope

This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.

Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable.

NOTE 1 For performance requirements, see Clause 9.

This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.

NOTE 2 For validation requirements, see 9.7.

The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.

The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.

The qPCR methods do not give any information about the physiological state of the Legionella.

Best Sellers From Food and Agriculture Sector

GSO 150-2:2013
 
Gulf Standard
Expiration dates for food products - Part 2 : Voluntary expiration dates
GSO 150-1:2013
 
Gulf Technical Regulation
Expiration dates for food products -Part 1 : Mandatory expiration dates
GSO 2055-1:2015
 
Gulf Technical Regulation
HALAL FOOD - Part 1 : General Requirements
GSO 1016:2015
 
Gulf Technical Regulation
Microbiological Criteria for Foodstuffs

Recently Published from Food and Agriculture Sector

GSO 1871:2022
 
Gulf Technical Regulation
Instant noodles
GSO 1003:2022
 
Gulf Technical Regulation
Rice Kernels
GSO 1019:2022
 
Gulf Technical Regulation
Edible olive oil and olive pomace oil
GSO CODEX STAN 301:2022
CODEX STAN 301:2011 
Gulf Technical Regulation
Standard for Edible Sago Flour