GSO ISO 17601:2017

ISO 17601:2016
Gulf Standard   Current Edition · Approved on 03 October 2017

Soil quality -- Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

GSO ISO 17601:2017 Files

English 31 Pages
Current Edition Reference Language

GSO ISO 17601:2017 Scope

This International Standard specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups. It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

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