GSO ISO/TS 23893-2:2013

ISO/TS 23893-2:2007

Gulf Standard Current Edition · Approved on 01 April 2013

Water quality - Biochemical and physiological measurements on fish - Part 2: Determination of ethoxyresorufin-O-deethylase (EROD)

GSO ISO/TS 23893-2:2013 Files

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GSO ISO/TS 23893-2:2013 Scope

This part of ISO 23893 specifies a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method. It applies to fish that are sampled in their natural environment (fresh water or salt water) or exposed to substances or effluents in a laboratory. This method is applicable for EROD values greater than or equal to 1 pmol/(min·mg) of proteins. A higher sensitivity may be achieved by using a cell (test tube) procedure